The show focuses primarily on three particular puppies; Lucky, TV addict leader and unconventional hero, Rolly, his fat cheerful laid-back and always-hungry brother, and Cadpig, their intellectual and fast-talking sister, who is the runt of the family. The three siblings are often joined by Spot, a chicken who wants to be a dog and a member of the Bark Brigade.Early promotional material had listed dictatorial Penny and mischievous Patch as two other puppy cast members, but the highlighted puppies were whittled down to three. The series is set on the Dalmatian Plantation, the farm where the dalmatians relocated after the end of the film; one episode features the dalmatians running away to return to the now-abandoned London flat where they were born, shortly after the events of the film.
Other puppies appearing in the show have included the fearless, three-legged Tripod, the friendly dim-witted Dipstick, the constantly urinating Wizzer, the fashion-consciousdivaTwo-Tone, and the Sheepdog mix, Mooch, who serves as the farm's bully. Penny and Patch also make occasional appearances, though with different personalities than originally promoted. The show seems to be a blending of both the animated film and the live-action film, so the potential exists for other puppies from the films to appear, especially Freckles, Pepper, Jewel, and Fidget.